miscript rt ii kit (Qiagen)

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Qiagen miscript rt ii kit
Miscript Rt Ii Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+ii+kit/Reverse+transcriptase/us12447172-220-9-13
Average 99 stars, based on 7641 article reviews

miscript rt ii kit - by Bioz Stars, 2026-10

99/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: MiRNA signature expression in cancer
Article Snippet: .. MicroRNA Detection and Quantitative PCR Analysis: cDNA will be synthesized using the miScript RT II kit (QIAGEN) in batches of 5 cases with their 10 matched controls. ..

Article Title: DROSHA, DICER and Damage-Induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break
Article Snippet: Immunoprecipitation was conducted overnight with ten μg of anti-BMI1 (Bethyl; #A301-694A) or normal rabbit IgG (Cell Signaling; #2729) as a control. .. DDRNAs were reverse transcribed using miScript RT II Kit (Qiagen; #1038703) and analyzed by qPCR with QuantiTect SYBR® Green PCR kit (Qiagen; #204145). ..

Synthesized:

Article Title: MicroRNA-194 regulates parasitic load and IL-1β-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis
Article Snippet: .. Briefly, cDNA was synthesized using a miScript RT II kit (Qiagen, MD, USA), as recommended by the manufacturer. .. Next, RT-qPCR was performed using a commercially available primer specific for Canis familiaris miR-194 (Qiagen, MD, USA).

Reverse Transcription:

Article Title: LncRNA LYPLAL1 , miR-204-5p , and SIRT1: novel signatures for risk assessment of diabetic macrovascular complications
Article Snippet: The quantity and purity of the RNA were assessed using a NanoDrop-2000 spectrophotometer from Thermo Scientific (USA). .. One microgram of RNA was used in each reverse transcription reaction, which was carried out following the steps outlined by QIAGEN for the miScript RT II kit (GmbH, Hilden, Germany). .. The miScript SYBR Green PCR kit was utilized on a StepOne real-time PCR instrument that was manufactured by Applied Biosystems in the United States.

Article Title: Use of the miR-27a-5p microRNA for treating
Article Snippet: .. For this reason, cDNA synthesis was performed using the miScript RT II® kit (Qiagen) containing reverse transcriptase and poly(A)-polymerase, a nucleic acid mix (containing dNTPs, rATP, oligo-dT primers and synthetic RNA serving as an internal control), in order to evaluate the RT performance and the “miScript HiSpec” buffer allowing selective conversion of mature miRNAs into cDNA. ..

SYBR Green Assay:

Polymerase Chain Reaction:

Isolation:

Article Title: Enhanced Extracellular Vesicle Cargo Loading via microRNA Biogenesis Pathway Modulation.
Article Snippet: Extracellular vesicles (EVs) are physiological vectors for the intercellular transport of a variety of molecules.. Among these, small RNAs, and especially microRNAs (miRNAs), have been identified as prevalent components, and there has thus been a robust investigation of EVs for therapeutic miRNAs delivery.. However, intrinsic levels of EVassociated miRNAs are generally too low to enable efficient and effective therapeutic outcomes.

Produced:

cDNA Synthesis:

Article Title: Reciprocal regulation between DNMT3A/3B and microRNAs miRs-299-3p/-30e is a causal factor for the downregulation of microRNAs targeting androgen receptor in prostate cancer
Article Snippet: .. A mirNome miRNA profiling kit (System Biosciences) for tissue samples and miScript RT II Kit (Qiagen) for cell lines were used for cDNA synthesis. .. Subsequent qRT-PCR was conducted using specific primers for miR-34e-5p, miR-299-3p and miR-30e and 3 small nuclear RNAs (U6 snRNA, RNU43 snoRNA, RNU1A snRNA) (System Biosciences) as internal controls, and 2X Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific).

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